Journal: Translational Andrology and Urology
Article Title: Silencing SPP1 in M2 macrophages inhibits the progression of castration-resistant prostate cancer via the MMP9/TGFβ1 axis
doi: 10.21037/tau-24-127
Figure Lengend Snippet: SPP1 + M2 macrophages promote the progression of prostate cancer. (A) Cell scratch experiments were conducted to verify the effect of M0/M2 macrophages and M2 macrophages knocking down SPP1 conditioned medium on the migration ability of PC3 cells. Right is the quantified result of the scratch area. (B) Representative images show the effects of M0/M2 and M2 knockdown of SPP1 conditional medium on the invasion and migration ability of PC3. The quantitative results of transmembrane cells were stained with crystal violet and shown on the right, with each result repeated 3 times and 3 randomly selected fields of view. (C) Representative BLI images of mice after orthotopic implantation of luciferase-labeled RM1 cells on day 3 and day 33 (n=4:4). (D) Prostate fluorescence of surviving mice on day 33. (E) The volume of prostate tumors in vitro in the inhibitor and control group mice was measured using the formula V = L × W 2 /2, where L represents the long diameter and W represents the short diameter. (F,G) The volume and mass of isolated prostate tumors separately. (H) Representative image of the immunohistochemical staining of SPP1, CD206, and Ki67 in two groups of mice tumor tissues. All data are the mean ± standard deviation. ANOVA was used in (B), two-tailed unpaired Student’s t -test was used in (A,E,F,G). *, P<0.05; **, P<0.01; ***, P<0.001. ns, no significant; CM, conditioned medium; SPP1, secreted phosphoprotein 1; KD, knockdown; CMC, carboxymethyl cellulose; TAA, thioacetamide; Ave, average; si, small interfering; BLI, bioluminescence imaging; ANOVA, analysis of variance.
Article Snippet: The human PCa cell lines PC3 and the human monocytic leukemia cell line THP-1, as well as the mouse PCa cell line RM1 were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA).
Techniques: Migration, Knockdown, Staining, Luciferase, Labeling, Fluorescence, In Vitro, Control, Isolation, Immunohistochemical staining, Standard Deviation, Two Tailed Test, Imaging
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